Table 1.
C. elegans strains and plasmids used in this study
| Transgenic strains | Genotypes | Proteins expressed | References |
|---|---|---|---|
| wLP 903 | leaSi41[Pmei-2::sgfp::mei-2 WT recoded + unc-119(+)]II; unc-113(ed3) III | sGFP::MEI-2R WT | This study |
| wLP 980 | leaSi42[Pmei-2::sgfp::mei-2 K77A K78A recoded + unc-119(+)]II; unc-113(ed3) III | sGFP::MEI-2R K77A K78A | This study |
| wLP 982 | leaSi43[Pmei-2::sgfp::mei-2 K77D K78D K274D K275D recoded + unc-119(+)]II; unc-113(ed3) III | sGFP::MEI-2R K77D K78D K274D K275D | This study |
| wLP 1045 | leaSi44[Pmei-2::sgfp::mei-2 K77D K78D recoded + unc-119(+)]II; unc-113(ed3) III | sGFP::MEI-2R K77D K78D | This study |
| wLP 1047 | leaSi45[Pmei-2::sgfp::mei-2 K274A K275A recoded + unc-119(+)]II; unc-113(ed3) III | sGFP::MEI-2R K274A K275A | This study |
| wLP 1050 | leaSi46[Pmei-2::sgfp::mei-2 K274D K275D recoded + unc-119(+)]II; unc-113(ed3) III | sGFP::MEI-2R K274D K275D | This study |
| wLP 1067 | leaSi47[Pmei-2::sgfp::mei-2 K77A K78A K274A K275A recoded + unc-119(+)]II; unc-113(ed3) III | sGFP::MEI-2R K77A K78A K274A K275A | This study |
| wLP 1087 | leaSi48[Pmei-2::sgfp::mei-2 K246A K249A recoded + unc-119(+)]II; unc-113(ed3) III | sGFP::MEI-2R K246A K249A | This study |
| wLP 1092 | leaSi49[Pmei-2::sgfp::mei-2 K246D K249D recoded + unc-119(+)]II; unc-113(ed3) III | sGFP::MEI-2R K246D K249D | This study |
| wLP 1127 | leaSi50[Pmei-2::sgfp::mei-2 K8A K9A K77A K78A K274A K275A recoded + unc-119(+)]II; unc-113(ed3) III | sGFP::MEI-2R K8A K9A K77A K78A K274A K275A | This study |
| wLP 1130 | leaSi51[Pmei-2::sgfp::mei-2 K8D K9D K77D K78D K274D K275D recoded + unc-119(+)]II; unc-113(ed3) III | sGFP::MEI-2R K8D K9D K77D K78D K274D K275D | This study |