FIG. 2.
The type 1 and type 2 fimbrial subunits are encoded by single gene copies in Actinomyces isolates. Southern blot hybridization of chromosomal DNA from A. naeslundii isolates with the fimP type 1 central DNA probe (left panel: lane 1, strain B-1-K; lane 2, P-1-K; lane 3, T14V; and lane 4, LY7), the fimA type 2:1 central DNA probe (middle panel: lane 1, strain Pn-22-E; lane 2, Pn-6-GA; lane 3, P-5-N; and lane 4, ATCC 12104T), and the fimA type 2:2 central DNA probe (right panel: lane 1, strain P-1-K; lane 2, P-1-N; and lane 3, LY7). Chromosomal DNA was digested with BamHI prior to hybridization with either type 1 or type 2:1 DNA probes and with PstI prior to hybridization with the type 2:2 DNA probe. The sizes (in kilobases) of HindIII-digested bacteriophage lambda DNA standards (lanes kb) are indicated on the right.