Fig. 4. Impairments of social interaction in znf536 KO zebrafish.

A Representative heatmap of the social interaction assay. The red color indicates increased dwell time and blue indicates decreased. Solid lines indicate a transparent divider and dashed lines indicate the very close zone (2 cm from the social cue region). B Quantified data for duration within the very close zone. n = 11 for WT, n = 19 for Het, and n = 23 for KO. Data was presented as mean ± standard error of the mean (S.E.M.). Statistical significance was determined by Kruskal–Wallis test with post-hoc Dunn’s multiple comparisons test. ns, no significance; p**** < 0.0001. C Representative image from the shoaling assay. Individual adult fish were pointed by arrows. D Quantified data for the average of the inter-fish distance of the shoaling assay. The test was performed 3 times. For each trial, 3 fish of each genotype were used.