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. 2024 Feb 8;14:3282. doi: 10.1038/s41598-024-52852-4

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PCoA of the intervention groups using the Jaccard, Bray–Curtis, and unweighted UniFrac distance metrics based on feature level. The data points represent individual samples (triangles for 0-week and circles for 12-week). Values in parentheses show the percentage of total variance explained by each axis. Statistical significance was determined using PERMANOVA and confirmed using PERMDISP; Jaccard (p = 0.001), Bray–Curtis (p = 0.002), and unweighted UniFrac (p = 0.001).