TABLE 2.

ORFs identified by nucleotide sequencing of a PAI of E. coli CFT073

ORFa	Coordinatesb	Homolog	Description of product encodedc	Probabilityd	Accession no. of homolog
L1	85–1155	dadX	Alanine racemase	1.3e−254	P29012
L2	2822–1212 C	f536	536-aa ORF product of E. coli	0.0	AE000217
L3	3957–3043 C	f304	304-aa ORF product of E. coli	3.3e−216	AE000217
L4	4165–4668	o241	241-aa ORF product of E. coli	4.8e−113	AE000217
L5	7879–5909 C	prrA	TonB-dependent outer membrane receptor	0.0	U85771
L6	8762–7905 C	modD	Molybdenum transport protein	1.2e−200	U85771
L7	9571–8759 C	orf2	Similar to H. influenzae yc73 protein	1.6e−112	U85771
L8	10203–9705 C	fepC	Ferric enterobactin transport ATP-binding protein	1.5e−31	F64113
Gap GL (∼2 kb)
f1569	12502–16056		1,569-aa ORF product of E. coli		ECAE000350
o109	16749–16420 C		109-aa ORF product of E. coli		ECAE000349
o105	17087–16770 C		105-aa ORF product of E. coli		ECAE000349
o160	17816–17334 C		160-aa ORF product of E. coli		ECAE000349
o152	18283–17825 C		152-aa ORF product of E. coli		ECAE000349
o567	20882–19181 C		567-aa ORF product of E. coli		ECAE000349
Gap LH (∼1 kb)
hlyC	21357–21869		Chromosomal hemolysin C		M10133
hlyA	21881–24952		Chromosomal hemolysin A		M10133
hlyB	25023–27146		Chromosomal hemolysin B		M10133
hlyD	27165–28601		Chromosomal hemolysin D	2.7e−285	Y13891
HP1	29731–28913 C		IS600 hypothetical 31-kDa protein	7.0e−195	P16940
HP2	30069–29767 C		IS600 hypothetical 11-kDa protein	2.1e−60	P16939
HP3	30172–30462		Unknown protein of plasmid Ti	2.1e−07	M25805
HP4	30547–30894		Hypothetical 15.6-kDa protein	7.7e−42	P50359
Gap HP (∼1 kb)
papG	34008–33001 C		P-pilus F13 tip protein		X61239
papF	34555–34052 C		P-pilus F13 tip protein		X61239
papE	35151–34630 C		P-pilus F13 tip protein		X61239
papK	35709–35176 C		P-pilus F13 tip protein		X61239
papJ	36300–35719 C		P-pilus F13		X61239
papD	37053–36337 C		P-pilus F13		X61239
papC	39649–37139 C		P-pilus F13		X61239
papH	40295–39708 C		P-pilus F13		X61239
papA	40925–40359 C		P-pilus F13		X61239
papB	41446–41132 C		P-pilus F13		X61239
papI			P-pilus F13		X61239
R15	43029–42643 C		Putative transposase of E. coli	1.6e−73	U06468
R14	43529–43174 C		Transposase (IS629)	8.6e−74	P16942
R13	43855–43529 C		12.7-kDa protein of E. coli	3.1e−51	U06468
R12	45171–44695 C		Transposase (ISAE1)	3.4e−06	A47041
R11	45575–45887		Possible precursor polypeptide	0.88	X00729
R10	45884–46144		Hypothetical 8.6-kDa protein in dinG/rarB 3′ region	6.2e−06	P41038
R9	46789–47217	f200	200-aa ORF product of E. coli	3.0e−08	AE000137
R8	47771–47503 C		Neurotensin receptor	0.32	P30989
R7	48288–48534		Maltopentaose-forming amylase	0.038	D10769
R6	49286–50752		Transposase of Chelatobacter heintzii	3.7e−06	L49438
R5	50914–51501	orfB	Hypothetical protein B of Bacillus	1.1e−46	S23889
R4	54177–52168 C		Exogenous ferric siderophore receptor	2.2e−162	U56084
Gap GR (<1 kb)
R3	56231–55869 C		β-Cystathionase	7.1e−100	U65013
R2	57811–56231 C	malX	Phosphotransferase system maltose- and glucosespecific IIabc component	3.5e−155	P19642
R1	58724–57843 C		sacpa operon antiterminator	2.6e−53	P26212
f447	60267–58885 C

^aFor positions of ORFs, see Fig. 1. 

^bBase pair number beginning at left junction of PAI; C indicates that ORF is found on the complementary strand. 

^cDescription of the best homolog. aa, amino acid. 

^dProbability value (P) of <0.05 is considered significant.