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. 2024 Jan 26;12:1342590. doi: 10.3389/fbioe.2024.1342590

FIGURE 4.

FIGURE 4

Effect on osteogenic differentiation of encapsulated ADSC: (A). ALP staining of ADSC after 7 days of osteogenic induction and ARS after 7 days and 14 days of osteogenesis induction; (B). ALP enzyme activity of ADSC after 7 days of osteogenic induction; (C). Semi-quantitative analysis of ARS staining; (D–F): Relative expression levels of Runx2 (D), ALP (E) and OCN (F); (G). Immunofluorescence staining of Runx2; (H). Semi-quantitative analysis of fluorescence intensity of Runx2. (n = 3, *p < 0.05, **p < 0.01).