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. 2024 Feb 8;150(2):83. doi: 10.1007/s00432-023-05558-5

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© The Author(s) 2024

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Fig. 2 — Characteristics of circBRIP1. a Sanger sequencing to identify cyclization sites; b qRT-PCR product length of circBRIP1 was confirmed by agarose gel electrophoresis (95 bp); c stability of circBRIP1 confirmed by RNase R digestion assay; d Actinomycin D test demonstrated the half-life of circBRIP1 in NCI-H1299 cell; e standard curves of plasma circBRIP1 and 18S in a tenfold serial dilution to demonstrate the linearity; f, g stability of circBRIP1 under room temperature incubation time or multiple freeze–thaw cycles; h specificity of PCR products by the melting curve