FIG. 3.

Reaction of MAb 10B6 with human cardiac myosin by Western immunoblot analysis. MAb 10B6 (20 μg/ml) (lane 1) and a control antibody were reacted with purified myosin by a Western immunoblot analysis. Similar results were obtained with rabbit skeletal (data not shown) and human cardiac myosin. Lanes 2 and 3, no reactivity of myosin with normal mouse serum (1:100) and an isotype control, respectively; lane S, amido black protein stain of the 200-kDa band of myosin heavy chain. MWS, molecular size standards.