FIG. 4.

Inhibition of the binding of MAb 10B6 to myosin with M5 peptides. MAb 10B6 (20 μg/ml) was preincubated with 100 to 0.05 μg of M5 peptide C3 or C2A per ml before being reacted with skeletal myosin by ELISA. Percentage of inhibition was calculated based on the reaction of MAb 10B6 without inhibitor and diluted in the buffer used in the inhibition. Results were identical with those for purified rabbit skeletal and human cardiac myosin. At concentrations of 1 to 500 μg/ml, M5 peptide C1C2 showed no inhibition of MAb 10B6.