TABLE 1.
Reactivity of MAb 10B6 with overlapping synthetic peptides of streptococcal M5 protein
| Peptidea | Sequence | Residues | Reactivity of MAb 10B6 (20 μg/ml) |
|---|---|---|---|
| NT1 | AVTRGTINDPQRAKEALD | 1–18 | 0.05 |
| NT2 | KEALDKYELENHDLKTKN | 14–31 | 0.00 |
| NT3 | LKTKNEGLKTENEGLKTE | 27–44 | 0.00 |
| NT4 | GLKTENEGLKTENEGLKTE | 40–58 | 0.00 |
| NT4–5 | GLKTEKKEHEAENDKLK | 54–70 | 0.00 |
| NT5 | KKEHEAENDKLKQQRDTL | 59–76 | 0.00 |
| NT5–6 | DKLKQQRDTLSTQKETLE | 67–84 | 0.00 |
| NT6 | QRDTLSTQKETLEREVQN | 72–89 | 0.00 |
| NT7 | REVQNTQYNNETLKIKNG | 85–102 | 0.00 |
| NT8 | KIKNGDLTKELNKTRQEL | 98–115 | 0.00 |
| B1A | TRQELANKQQESKENEKAL | 111–129 | 0.00 |
| B1B | ENEKALNELLEKTVKDKI | 124–141 | 0.00 |
| B1B2 | VKDKIAKEQENKETIGTL | 137–154 | 0.00 |
| B2 | TIGTLKKILDETVKDKIA | 150–167 | 0.00 |
| B2B3A | KDKIAKEQENKETIGTLK | 163–180 | 0.00 |
| B3A | IGTLKKILDETVKDKLAK | 176–193 | 0.00 |
| B2B3B | DKLAKEQKSKQNIGALKQ | 189–206 | 0.00 |
| B3B | GALKQELAKKDEANKISD | 202–219 | 0.00 |
| C1A | NKISDASRKGLRRDLDAS | 215–232 | 0.06 |
| C1B | DLDASREAKKQLEAEHQK | 228–245 | 0.00 |
| C1C2 | AEHQKLEEQNKISEASRK | 241–258 | 0.00 |
| C2A | EASRKGLRRDLDASREAK | 254–271 | 1.62 |
| C2B | SREAKKQLEAEQQKLEEQ | 267–284 | 0.00 |
| C2C3 | KLEEQNKISEASRKGLRR | 280–297 | 0.00 |
| C3 | KGLRRDLDASREAKKQ | 293–308 | 1.64 |