Table 1.

Thermodynamic parameters of the interactions between CXCL12 and Ac-pep, frHMGB1 and frHMGB1-TL

	ITCa				MST
	Kd1 (μM)	Kd2 (μM)	∆H1 (kcal/mol)	∆H2 (kcal/mol)	Kd[NaCl]=20mM (μM)	Kd[NaCl]=150mM (μM)
Ac-pep	0.6 ± 0.1	0.1 ± 0.1	−10.4 ± 0.8	1.6 ± 0.7	0.6 ± 0.1b	5.0 ± 0.6b
frHMGB1	1.2 ± 0.4	7.8 × 10−3 ± 1.5 × 10−2	−1.4 ± 0.1	−2.1 ± 0.2	1.7 ± 0.2c	31.8 ± 1.4c
frHMGB1-TL	n.d.	n.d.	n.d.	n.d.	12.5 ± 5.5c	81.4 ± 6.8c

^aCXCL12 is the titrant, n = 2 independent replicates, error estimates from covariance matrix.

^bCXCL12 is the titrant, n = 3 independent replicates, values are mean ± standard deviation.

^cfrHMGB1 or frHMGB1-TL is the titrant, n = 3 independent replicates, values are mean ± standard deviation.

n.d. not detected.