FIG. 8.

RT-PCR of RNA isolated from ferrets treated in the presence or absence of DEX and Bactrim. Bactrim-treated DEX-immunosuppressed (DEX), control (none), and P. carinii-infected (PCP) ferret lungs and livers were amplified with γ-FBG and β-actin primer pairs (A). Total RNAs from the livers and lungs of P. carinii-infected ferrets were amplified with a gpA-specific primer pair as positive controls for the RT-PCR (B). In both panels, no template served as the negative control reaction. The molecular size markers are in 100-bp increments.