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RT‐qPCR analysis on multiple differentiation markers for the human intestinal epithelial monolayers. The expression of Lysozyme, Villin, and SLC11a2 showed significantly higher expression in the monolayer under ALI conditions. The other two markers (Muc2 and CHgA) also showed the same trend; however, overall expression remained low). The human intestine was used as control. (n = 3). *p < 0.05.
