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. 2024 Feb 8;30(2):e14599. doi: 10.1111/cns.14599

FIGURE 1.

FIGURE 1

Transformation of NHAs into TAAs under GSCs‐exos education. (A) Expression of GSCs markers, Nestin, CD133, and OCT4 in GSC11 and GSC23 cells. (B) Exosomes purified from the cell culture medium of GSCs. Expression of the positive markers of exosomes TSG101 and HSP70, as well as the negative marker calnexin, were detected by Western blot, and expression of these three markers was evaluated in the cell lysate of GSCs as well. (C) Transmission electron microscopy of exosomes derived from GSCs. Scale bars: 100 nm. (D) Size distribution analysis of GSCs‐derived exosomes. (E) QRT‐PCR analysis on TGF‐β, CD44, tenascin‐C, and CD274 expression in TAAs and NHAs. (F) TAAs had obvious morphological changes with enhanced proliferation. **p < 0.01, ***p < 0.001, and ****p < 0.0001.