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. 2024 Feb 8;30(2):e14599. doi: 10.1111/cns.14599

FIGURE 2.

FIGURE 2

APNG overexpression conferred TMZ resistance onto glioblastoma cells. (A) Schematics of glioblastoma cells/TAAs indirect co‐culture system. (B) The IC50 values of TMZ by CCK‐8 assay in SNB19 and SF295 cells. (C) SNB19 and SF295 cells were co‐cultured with TAAs for 5 days, then were exposed to TMZ for 48 h, followed by apoptosis analysis by flow cytometry. (D) After co‐culturing with TAAs, SNB19, and SF295, cells were treated with TMZ (300 μM for SNB19, 200 μM for SF295) for 48 h, then a colony formation assay was performed. Tumor cells under TMZ exposure alone without co‐culturing with TAAs were set as a control. (E) Western blot analysis of γ‐H2AX expression in SNB19 or SF295cells after culturing with TAAs for 5 days, then treated with TMZ (300 μM for SNB19, 200 μM for SF295) 48 h. β‐actin was set as the loading control. SNB19 and SF295 cells under TMZ exposure alone without co‐culturing with TAAs served as a control. (F) Protein levels of APNG in glioblastoma cells co‐cultured with TAAs were assayed with Western blot.  **p < 0.01, ***p < 0.001, and ****p < 0.0001.