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. 2024 Feb 8;30(2):e14599. doi: 10.1111/cns.14599

FIGURE 6.

FIGURE 6

Upregulation of intracellular ALKBH7 increased APNG expression of glioblastoma cells. (A) Western blot for protein level of ALKBH7 in glioblastoma cells after co‐culturing with TAAs. (B) qPCR analysis of ALKBH7 expression in SNB19 or SF295 cells transfected with si‐NC, si‐ALKBH7‐1, or si‐ALKBH7‐2, respectively. (C) Flow cytometry analysis of SNB19 or SF295 cells after co‐culturing with TAAs followed with ALKBH7 knockdown and TMZ exposure (300 μM TMZ for SNB19, 200 μM for SF295 cells) for 48 h. (D) Soft agar colony formation assay of SNB19 or SF295 cells after co‐culturing with TAAs followed with ALKBH7 knockdown and TMZ exposure (300 μM TMZ for SNB19, 200 μM for SF295 cells) for 48 h. (E) Western blot of γ‐H2AX expression of SNB19 or SF295 cells after co‐culturing with TAAs followed with ALKBH7 knockdown and TMZ exposure (300 μM TMZ for SNB19, 200 μM for SF295 cells) for 48 h. (F) The protein level of ALKBH7 and APNG in SNB19 or SF295 cells after co‐culturing with TAAs followed with ALKBH7 knockdown. **p < 0.01, ***p < 0.001, and ****p < 0.0001.