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. 2023 Dec 1;52(3):1313–1324. doi: 10.1093/nar/gkad1157

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — The hTOP2β core can perform supercoil relaxation and decatenation. (A) Linear arrangement of domains within hTOP2β. (B) Activity of core and full-length hTOP2β enzymes on negatively supercoiled plasmid DNA in the presence (+) or absence (-) of ATP. Ratios refer to enzyme dimers:DNA. Nicked (N), relaxed (R), linearized (L), or supercoiled (SC) species are indicated. (C) Time course of hTOP2α and hTOP2β core enzymes acting on negatively supercoiled plasmid DNA. (D) Decatenation of kDNA by the hTOP2β core as titrated against different concentrations of DMSO. Enzyme (100 nM) was incubated with 250 ng of kDNA for 30 min at 37°C. Nicked (N), linearized (L) or supercoiled (SC) minicircle species as well as dimeric (D) and higher order catenated species are indicated.