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. 2023 Dec 1;52(3):1313–1324. doi: 10.1093/nar/gkad1157

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Headless hTOP2β has enhanced activity over the hTOP2β core but is more cleavage prone than full-length hTOP2β. (A) Relaxation activity of the hTOP2β core, headless hTOP2β (HL) and full-length (FL) hTOP2β on negatively supercoiled plasmid DNA in the presence (+) or absence (-) of ATP. Ratios refer to enzyme dimers:DNA. (B and C) Assays as per (A) but with products separated on agarose gels containing ethidium bromide (EtBr), having either been quenched only with SDS (B, irreversible and reversible products) or with EDTA followed by SDS (C, irreversible products only). Nicked (N), relaxed (R), linearized (L), or supercoiled (SC) species are indicated.