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. 2023 Dec 5;52(3):1374–1386. doi: 10.1093/nar/gkad1136

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — The effect of tRNA modifications on decoding by M. mycoides tRNA^Gly UCC. (A) The M. mycoides tRNA^Gly UCC carrying different sets of modifications were tested for their capability to decode the synonymous glycine codons. [³⁵S]-Met labelled peptides were quantified and normalized to the peptide yields resulting from the GGA containing mRNA. Open circles indicate datapoints shown in the representative gels. (B) Depletion of the three E. coli tRNA^Gly isoacceptors within E. coli tRNA bulk was rescued with the differently modified M. myocides tRNA^Gly. The fLuc activities were normalized to the unmodified tRNA sample. (A, B) Mean of individual datapoints with standard deviation is shown. Significance was tested by ANOVA ((A) 0.000424 (left); 0.000782 (right); (B) 0.000722) followed by Dunnett's test (0.001 ‘***’, 0.01 ‘**’, 0.05 ‘*’, >0.05 ‘ns’).