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. 2023 Dec 5;52(3):1374–1386. doi: 10.1093/nar/gkad1136

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — The effect of m⁶A37 on decoding. (A) Secondary structure of the hybrid modified E. coli tRNA^Gly UCC carrying the native T-arm modifications T54 and P55 (shaded in blue) and the M. mycoides tRNA modification m⁶A at position 37 (shaded in red). (B) Depletion of the three E. coli tRNA^Gly isoacceptors within E. coli tRNA bulk was rescued with the differently modified E. coli tRNA^Gly UCC. Datapoints represent measurements of technical duplicates of individual experiments. The fLuc activity was normalized to the unmodified tRNA sample. Mean of individual datapoints with standard deviation is shown. Significance was tested by ANOVA (not significant).