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. Author manuscript; available in PMC: 2024 Feb 9.
Published in final edited form as: Cell Rep. 2023 Dec 12;42(12):113571. doi: 10.1016/j.celrep.2023.113571

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Bacterial and virus strains
Bacillus subtilis strain 168 (trpC2) BGSC 1A1
1A1 speD::kan BGSC BKK29010
1A1 speE::kan BGSC BKK37500
1A1 amyE:PlysC- TilldcCWT-lacZ cat This study HS240
1A1 amyE:PlysC- TilldcCM1-lacZ cat This study HS241
1A1 amyE:PlysC- TilldcCM2-lacZ cat This study HS243
1A1 amyE:PlysC- TilldcCM3-lacZ cat This study HS244
BKK37500 amyE:PlysC- TilldcCWT-lacZ cat BGSC HS428
BKK29010 amyE:PlysC-TilldcCWT-lacZ cat BGSC HS433
1A1 amyE:PlysC- TilldcCM4-lacZ cat This study HS592
1A1 amyE:PlysC- TilldcCM5-lacZ cat This study HS593
1A1 amyE:PlysC- TilldcCM6-lacZ cat This study HS600
1A1 amyE:PlysC- TilldcCM7-lacZ cat This study HS601
1A1 amyE:PlysC- TilldcCM8-lacZ cat This study HS602
1A1 amyE:PlysC- TilconshypoWT-lacZ cat This study HS603
1A1 amyE:PlysC- TilconshypoM15-lacZ cat This study HS605
BKK37500 1A1 amyE:PlysC- TilconshypoWT-lacZ cat This study HS607
BKK29010 1A1 amyE:PlysC- TilconshypoM15-lacZ cat This study HS608
Chemicals, peptides, and recombinant proteins
[α−32P]UTP PerkinElmer Cat #: BLU507H250UC
[γ−32P]ATP PerkinElmer Cat #: BLU502Z500UC
1,3-Diaminopropane Sigma-Aldrich Cat #: D23602–25G
5′-Deoxy-5′-(methylthio)adenosine Sigma-Aldrich Cat #: D5011
Agmatine sulfate Sigma-Aldrich Cat#: A7127
Bis(3-aminopropyl)amine (nor spermidine) Sigma-Aldrich Cat#: I1006
Cadaverine Sigma-Aldrich Cat #: C8561
E. coli RNA Polymerase, Holoenzyme New England Biolabs Cat #: M0551
Ethyl-1,3-diaminopropane Santa Cruz Biotechnology Cat: #: sc-331624
L-Arginine Sigma-Aldrich Cat #: A5006
L-Ornithine hydrochloride Sigma-Aldrich Cat #: O-2375
N1-acetylspermidine (hydrochloride) Cayman Chemical Cat #: 9001535
N1-acetylspermine (hydrochloride) Cayman Chemical Cat#: 17919
N-Methyl-1,3-diaminopropane Sigma-Aldrich Cat#: 127027–25G
O-nitrophenyl-β-D-galactopyranoside (ONPG) Thermo Fisher Scientific Cat #: 34055
Propyl-1,3-diaminopropane Santa Cruz Biotechnology Cat #: sc-236089
Putrescine Sigma-Aldrich Cat #: 51799
Quick CIP New England Biolabs Cat #: M0525
RNase T1 (1 U/ml) Thermo Fisher Scientific Cat #: AM2283
S-(5′-Adenosyl)-3-thiopropylamine Sigma-Aldrich Cat#: 43713
S-(5′-Adenosyl)-L-methionine chloride dihydrochloride Sigma-Aldrich Cat #: A7007
Spermine Sigma-Aldrich Cat #: 85590
Spermidine trihydrochloride Sigma-Aldrich Cat #: S2501
T4 Polynucleotide Kinase New England Biolabs Cat #: M0201
TURBO DNase (2 U/ml) Thermo Fisher Scientific Cat #: AM2238
X-gal Cayman Chemical Cat#: 16495
Deposited data
Oceanobacillus damuensis RefSeq80 (Ref. #52; https://doi.org/10.1093/nar/gkv11989 ) NZ_LQNF01000010.1/43200–43335
Bacillus thermoamylovorans RefSeq80 NZ_CCRF01000060.1/8653–8516
Rummeliibacillus stabekisii RefSeq80 NZ_CP014806.1/1179153–1179287
Trichococcus sp. ES5 RefSeq80 NZ_FJND01000001.1/36715–36568
Lysinibacillus sp. BF-4 RefSeq80 NZ_JPUW01000046.1/6598–6731
Environmental DNA RefSeq80 BMHBC_202880/1413–1562
Trichococcus ilyis RefSeq80 NZ_FJNB01000014.1/83394–83245
Oceanobacillus massiliensis str. N’diop RefSeq80 NZ_HE610978.1/9297–9431
Trichococcus flocculiformis RefSeq80 FJMZ01000015.1/31932–32065
Viridibacillus arvi RefSeq80 NZ_LILB01000001.1/679934–679798
Sporosarcina sp. D27 RefSeq80 NZ_AZUC01000044.1/37858–37992
Ornithinibacillus californiensis RefSeq80 NZ_LDUE01000005.1/135850–135988
Ornithinibacillus contaminans RefSeq80 NZ_LDPV02000046.1/73086–73221
Oligonucleotides
See Table S1 for oligonucleotide sequences.
Recombinant DNA
pDG1661tsl Li et al.79 N/A
pDG1661tsl-TilldcCWT GenScript N/A
pDG1661tsl-TilldcCM1 GenScript N/A
pDG1661tsl-TilldcCM2 GenScript N/A
pDG1661tsl- TilldcCM3 GenScript N/A
pDG1661tsl-TilldcCM4 GenScript N/A
pDG1661tsl-TilldcCM5 GenScript N/A
pDG1661tsl-TilldcCM6 GenScript N/A
pDG1661tsl-TilldcCM7 GenScript N/A
pDG1661tsl-TilldcCM8 GenScript N/A
pDG1661tsl-TilconshypoWT GenScript N/A
pDG1661tsl-TilconshypoM15 GenScript N/A
Software and algorithms
ImageJ NIH https://imagej.nih.gov/ij/
Image Lab Bio-Rad https://www.bio-rad.com/en-us/product/image-lab-software?ID=KRE6P5E8Z
Prism 9 GraphPad https://www.graphpad.com/