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. 2024 Feb 9;30(2):e14618. doi: 10.1111/cns.14618

FIGURE 3.

FIGURE 3

EA inhibited the polarization of M1 microglia/macrophages in the ischemic cerebral cortex. (A) Immunofluorescence showed that EA reduced the number of Iba1+ CD86+ cells in the ischemic cerebral cortex. (B–E) After MCAO, the mRNA expression of pro‐inflammatory cytokines Ccl‐2, Il‐6, Tnf‐α, and Il‐1β increased. EA treatment suppressed this upregulation. (F, G) EA treatment inhibited the elevated protein levels of iNOS after MCAO. (H) Immunofluorescent staining revealed iba1+ and CD206+ cells in the ischemic cerebral cortex of the Sham group, MCAO group, and EA group. (I–L) Compared with the MCAO group, after EA treatment, there was no statistically significant difference in the mRNA expression levels of Tgf‐β, Il‐10, Cd206, and Arg‐1 secreted by M2 microglia/macrophages. *p < 0.05; **p < 0.01; ***p < 0.001; n = 4–8 per group.