Table 1. Discriminatory Metabolites Discovered by the Targeted Metabolomics Approach in 6-Month 5xFAD Transgenic Mice.

metabolite names	PubChem CID	chemical formula	metabolic pathway	retention time (min)	measured m/z	mass accuracy (ppm)	fold change	p value
sphinganine	91486	C18H39NO2	sphingolipid metabolism	9.0	302.3050	1.4	0.5	0.011
3-dehydrosphinganine	439853	C18H37NO2	sphingolipid metabolism	0.6	300.2892	1.7	1.5	0.028
l-valine	6287	C5H11NO2	valine, leucine and isoleucine biosynthesis	2.2	116.0703	2.4	0.5	0.005
d-erythro-3-Methylmalate	440892	C5H8O5	valine, leucine and isoleucine biosynthesis	0.8	147.0286	1.5	1.2	0.011
2-methylmaleate	643798	C5H6O4	valine, leucine and isoleucine biosynthesis	1.1	129.0179	2.0	1.2	0.009
l-leucine	6106	C6H13NO2	valine, leucine and isoleucine biosynthesis	1.3	130.0860	1.3	0.6	0.043
N-acetyl-l-aspartate	65065	C6H9NO5	alanine, aspartate and glutamate metabolism	0.8	174.0397	0.0	2.3	0.010
l-glutamine	5961	C5H10N2O3	alanine, aspartate and glutamate metabolism	1.3	147.0763	0.4	1.3	0.023
N-acetylisatin	11321	C10H7NO3	tryptophan metabolism	9.8	190.0497	1.3	0.6	<0.001
5-methoxyindoleacetate	18986	C11H11NO3	tryptophan metabolism	4.7	206.0812	0.0	2.2	0.018
2-aminophenol	5801	C6H7NO	tryptophan metabolism	3.4	108.0441	3.2	0.7	0.026
(R)-(indol-3-yl)lactate	676158	C11H11NO3	tryptophan metabolism	5.1	204.0657	1.0	1.4	0.026
indoxyl	50591	C8H7NO	tryptophan metabolism	3.5	134.0599	1.4	1.8	0.038
6-hydroxykynurenate	440752	C10H7NO4	tryptophan metabolism	3.7	204.0293	0.9	2.1	0.015
leukotriene B4	5280492	C20H32O4	arachidonic acid metabolism	7.3	337.2368	1.9	0.8	0.027
leukotriene E4	5280879	C23H37NO5S	arachidonic acid metabolism	4.7	438.2293	3.5	0.4	0.029
20-HETE	5283157	C20H32O3	arachidonic acid metabolism	10.5	319.2273	1.8	0.8	0.033
arachidonic acid	444899	C20H32O2	arachidonic acid metabolism	10.2	305.2472	1.0	0.8	0.050