FIG. 2.

Northern blot analysis of HLA-B27 mRNA in infected JT1 cells. JT1 cells were either mock infected or infected with the invasive strain S. flexneri M90T. At indicated times postinfection, total RNA was extracted, separated by electrophoresis on an agarose-formaldehyde gel, and transferred to a membrane. The membrane was sequentially hybridized with a 0.5-kb HLA-B7 probe which recognizes HLA-B27 RNA and then with a GAPDH probe. The cell line 7.3.13, expressing the human β2m only, was used as a control. The HLA-B27 transcript is designated by an arrow.