FIG. 3.

FACS analysis of total HLA-B27 expression in JT1 cells. JT1 cells were either mock infected (A) or infected with the invasive strain S. flexneri M90T for 2.5 h (B) and permeabilized before labeling with MAbs B1.23.2, ME1, and HC10. Infection was controlled by using a polyclonal antibody (FlexV) raised against S. flexneri serotype 5 (C). Specific staining in noninfected and infected cells is represented by light and bold lines, respectively; nonspecific staining is represented by dotted lines. Fluorescence intensity is expressed on the FL1 x axis, and the relative number of cells is shown on the y axis.