Table 3.

Structure Properties of Variant Proteins

Variants	Wild-Type	E72K	Q80H	R102W	S134P	Y151H	G171E	R182C	R197H	R200C	P203L	R213W	R213Q
hbnumsp	427	401	396	395	394	391	416	397	399	391	391	379	388
hbnumsp diff	0.00%	−6.09%	−7.26%	−7.49%	−7.73%	−8.43%	−2.58%	−7.03%	−6.56%	−8.43%	−8.43%	−11.24%	−9.13%
hbnumpp	100	105	104	116	106	99	100	103	108	101	101	100	102
hbnumpp diff	0.00%	5.00%	4.00%	16.00%	6.00%	−1.00%	0.00%	3.00%	8.00%	1.00%	1.00%	0.00%	2.00%
SASA	99.26	104.36	97.3	98.98	104.68	97.98	100.46	94.23	100.09	97.21	98.42	97.75	100.7
SASA diff	0.00%	5.13%	−1.98%	−0.28%	5.46%	−1.29%	1.21%	−5.08%	0.84%	−2.07%	−0.85%	−1.52%	1.45%
Hydrophilic area	9215.928	9141.156	8827.822	9097.777	9138.324	9009.037	9411.617	8974.289	8930.672	8719.049	9201.594	8859.148	9176.891
Hydrophilic area diff	0.00%	−0.81%	−4.21%	−1.28%	−0.84%	−2.24%	2.12%	−2.62%	−3.10%	−5.39%	−0.16%	−3.87%	−0.42%
Hydrophobic area	759.629	924.825	810.795	808.761	953.833	740.211	812.356	642.217	794.462	694.482	669.083	709.807	801.754
Hydrophobic area diff	0.00%	21.75%	6.74%	6.47%	25.57%	−2.56%	6.94%	−15.46%	4.59%	−8.58%	−11.92%	−6.56%	5.55%
RMSD	0.22	0.26	0.21	0.22	0.25	0.23	0.22	0.23	0.23	0.22	0.22	0.23	0.23
RMSD diff	0.00	0.05	−0.01	0.00	0.03	0.02	0.00	0.02	0.02	0.01	0.00	0.01	0.01
Delta_delta_G	−0.27	1.16	3.08	0.49	2.79	3.32	0.06	3.44	1.36	1.32	25.41	3.76	−2.21
Grantham distance	0.00	56.00	24.00	101.00	74.00	83.00	98.00	180.00	29.00	180.00	98.00	101.00	43.00
Residue accessibility	0.00	104.50	27.12	41.61	8.82	1.00	58.43	88.16	61.88	39.72	0.12	0.00	0.00
Alpha	0.00	5.38	5.98	3.59	5.98	5.38	3.59	2.39	4.19	4.19	1.79	0.00	4.19
Beta	38.92	33.53	37.12	35.32	35.32	36.52	35.92	32.33	31.13	35.92	38.32	33.53	40.71
Other	61.07	61.07	56.88	61.07	58.68	58.08	60.47	65.26	64.67	59.88	59.88	66.46	55.08
Beta diff	0.00	−0.14	−0.05	−0.09	−0.09	−0.06	−0.08	−0.17	−0.20	−0.08	−0.02	−0.14	0.05
Disulfide bond	2 (Cys63-Cys219, Cys 110-Cys142)

diff, difference.