A) Resection confirmation of various tumors in euthanized mice. In all cases the primary tumor site is highlighted by the solid arrow, with remnant lesions highlighted by the dotted arrow. In the PC3-PSMA cohort, resection was performed twice on one mouse to completely resect all tumor areas (1, 2). B) SNR quantification of all resected lesions, with sufficient contrast achieved in all cases (>5dB threshold). C) CNR quantification of all resected lesions, in all cases sufficient CNR was achieved (>3dB threshold) for all primary tumor sites. In all cases the mean, SD, and each replicate (dots, n=4 mice per tumor line, 5 dots include secondary sites) are shown for all tumor lines. Statistical comparisons were not performed as all tumors fulfilled SNR and CNR thresholds. D) H&E staining of tumor areas removed during resection, images shown at 20x magnification. All fluorescent areas were confirmed to be tumorous as characterized by the high density of nuclei. For the remnant PC3-PSMA tumor (2) removed during R2, this was determined as residual primary tumor tissue below the skin surface.