Figure 8. Non-invasive, non-contact and ambient light-resistant wound monitoring through commercially available bandages via SWIRFI (>1300nm) and CJ215.

A) Schematic representing the experimental protocol including the incision, suturing, injection, and imaging timepoints. Mice received a second injection once the wound had healed and were then imaged every 24 hrs from 1–48 hrs post this injection. Made with Biorender.com. B) Representative visible light and SWIRFI (>1300 nm) images without a bandage 2 hrs post-surgery and CJ215 injection. C) The same mouse as in A but with a hydrogel bandage place over the wound area. D) Representative images at 240h post-surgery and the rst injection of CJ215 highlighting no remnant wound fluorescence (>1300nm). E)Representative images at 384h post-surgery and 48h post the second injection of CJ215 highlighting no wound area uptake (wound has completely healed) F) Contrast quantification from all mice at all investigated wavelengths (>900, >1100 and >1300 nm cutoffs) with (solid lines) and without bandage placement over wound area (dotted lines). Wound contrast peaks at 48h post-surgery and post injection, decreasing over time as the wound heals. Bandage application prevented wound delineation at 168 hrs. A 3dB CNR threshold (grey dotted line) was utilized as before. In all cases the mean and SD are shown from n=4 mice with mice being measured immediately before and after bandage application. >900nm video rate imaging is shown in Supplemental Video 4 with all Visible, >900, >1100 and >1300nm images of all mice shown in Supplemental Figures#-#.