Figure 6.

Mice with H. pylori SS1+MNU-induced GC were supplemented with Bu (Mnu+bu) from 30 weeks to 52 weeks (−2 + 52 w). (a-b) Macroscopic observations of the stomachs, (c) tumor numbers, and (d) maximum tumor volumes were compared between the MNU and MNU+Bu groups (−2 + 52 w). (e) HE staining and (f) Immunohistochemical staining of the tumor-associated markers Ki-67 and CK-7, and GPR109A, HOPX, and IFN-γ in gastric tissues from the MNU and MNU+Bu groups. (g-j) Quantification of the results of (f). n = 5 mice/group, cages = 2 to 3/group, cages = 2 to 3/group. Data indicate the mean ± SD. *p < .05, **p < .01, and ***p < .001, by 2-tailed Student’s t test or one-way ANOVA.