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. 2024 Feb 6;16(1):2307542. doi: 10.1080/19490976.2024.2307542

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© 2024 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 8. — (a) Flow cytometry analysis of the proportions of CD3⁺ T, CD4⁺ T, CD8⁺ T, CD25⁺ FOXP3⁺ Treg cells, and DCs cells in mouse peripheral blood in the MNU and MNU+Bu groups (−2 + 52 w). (b) Quantitative statistical analysis of the fluorescence colocalization of (c). Positive cell rate (%). (c) Immunofluorescence staining showing the co-expression of CD8 and IFN-γ in the two groups. (d-e) Flow cytometry analysis of the proportions of DCs and CD3⁺ T, CD4⁺ T, and CD8⁺ T cells in mouse gastric tumor tissues and peripheral blood in the different groups (MNU+HF, MNU+Bu, MNU, MNU+GPR109A^−/−, MNU+GPR109A^−/−+Bu) (−2 + 38 w). (f) The co-expression of CD8 and IFN-γ in the five groups. (g) Quantitative statistical analysis of the fluorescence colocalization of (F). Positive cell rate (%). (h) Quantitative statistical analysis of the fluorescence colocalization of (I). Positive cell rate (%). (i) The co-expression of CD8 and IFN-γ in the groups (F-N, F-CA, F-MNU) (−6 + 36 w). (j-k) Flow cytometry analysis of the proportions of DCs and CD3⁺ T, CD4⁺ T, and CD8⁺ T cells in mouse gastric tumor tissues and peripheral blood in the three (F-N, F-CA, F-MNU) groups. n = 4 to 6 mice/group, cages = 2 to 3/group, cages = 2 to 3/group. Data indicate the mean ± SD. *p < .05, **p < .01, and ***p < .001, by 2-tailed Student’s t test or one-way ANOVA.