TABLE 1.
Virion production by Apr gene-marked H-19B lysogens
| Strain | Phage titer (PFU/104 CFU)a
|
Fold inductionc | |
|---|---|---|---|
| Before mitomycin treatment | After mitomycin treatmentb | ||
| MC4100 H-19B | 4 | 25 × 104 | 6.3 × 104 |
| MC4100 H-19B-Ap1 | 11 | 64 × 104 | 5.8 × 104 |
| MC4100 H-19B-Ap2 | 7 | 18 × 104 | 2.6 × 104 |
Serial dilutions of chloroform-treated supernatants were plated on E. coli C600.
Cultures with an optical density at 600 nm of 0.5 were treated with mitomycin C at 250 ng/ml for 4 h.
Calculated by dividing the number of PFU after mitomycin C treatment by the number of PFU prior to mitomycin C treatment.