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. 2023 Sep 1;9(35):eadg7053. doi: 10.1126/sciadv.adg7053

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Fig. 5. — (A) HEK293 cells stably expressing Flag-tagged MAVI1 were subjected to immunoprecipitation and mass spectrometry (IP-MS) analysis. (B) MAVI1-associated proteins identified were analyzed in REPRINT database to exclude common contaminants. Proteins with SAINT ≥ 0.95 and FC-B ≥ 4 were considered as specific binding. (C and D) HEK293 cells were subjected to IP analysis with anti-MAVI1 (C) or MAVS (D) antibody followed by IB analysis. (E) HEK293 cells transfected with or without MAVS in the presence or absence of MAVI1 were subjected to examine the expression of IFNB1 (mean ± SEM, *P < 0.05, ***P < 0.001). (F) HEK293 cells transfected with or without Flag-tagged MAVS in the presence of GFP or GFP-tagged MAVI1 were subjected to IB analysis.