Figure 5.

Supplementation with the S7 consortium improves behavior in frail donor recipient mice.

Spatial reference memory and learning ability were assessed via Barnes Maze. Mice were trained for 3 d and each training day included three training trials, lasting 3 min to allocate the submerged platform spatially. A latency of 3 min was used as a cutoff for the success rate. a. Success rates of S7 recipient mice and their germ-free controls at each training time b. The success rate of frail donor recipient mice treated with S7 (Frail+S7) compared to their frail/healthy donor recipient controls c. Frail donor recipient mice treated with S7 species (Frail+S7) were significantly faster to reach the target hole than frail donor recipient controls on the test day (p = .03). Data were analyzed using a one-way ANOVA post hoc Holm-Sidak. Box plots include the median line and the box denotes the interquartile range. Whiskers indicate the 1.5 interquartile range. Dots represent the outliers. *P < .05, **P < .01. n = 9 (Germ-free), n = 10 (S7 recipient), n = 9 (Healthy donor recipient), n = 9 (Frail donor recipient), n = 10 (Frail + S7).