Table 1.
Target gene | Forward primer sequence | Reverse primer sequence | Original reference |
---|---|---|---|
| |||
RyR2 (exon 94–95) | CCATTCTGCACACCGTCATC | GAGCTACTTCCTTTTCTCGCT | Jung et al. (2012) |
LTCC (Cacna1c) (exon 46–47) | CTGTCCCCAAAGAGGGGCTT | TCGCTTCAGACATTCAAGGT | Jung et al. (2012) |
NCX1 (Slc8a1) (exon 10–11) | GAGACCTCGCTTCCCACTTC | ATGCCCAGGAAGACGTTCACC | Jung et al. (2012) |
Jph2 (exon 4–5) | AGGCTGGGGCCAAGAAAAAG | CGATGTTCAGCAGGATCACCA | Xu et al. (2012) |
pan-Bin1 (exon 5–5/6) | ACAATGACCTGCTCTGGATGG | GCGACTTGATGTCAGGGAACT | Gayi et al. (2018) |
Dnm2 (exon 3–4) | ACAGGAGATCGAAGTGGAGAC | GGTCGATGAGGGTCAGGTTCAA | Gayi et al. (2018) |
Mtm1 (exon 4–5) | GAGGCGCCACAAGTAGAGG | GAAGGCGTATCTTGTGAGGA | Gayi et al. (2018) |
Gene-specific primers were designed based on previously described primer pairs, for measuring mRNA levels of the following transcripts: RyR2, LTCC, Jph2, pan-Bin1, Dnm2 and Mtm1. Primer pair specificity was controlled via dissociation curve analysis.