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. Author manuscript; available in PMC: 2024 Sep 24.
Published in final edited form as: J Physiol. 2023 May 21;602(18):4461–4486. doi: 10.1113/JP284538

Table 1.

Primer pairs employed for quantitative RT-PCR

Target gene Forward primer sequence Reverse primer sequence Original reference

RyR2 (exon 94–95) CCATTCTGCACACCGTCATC GAGCTACTTCCTTTTCTCGCT Jung et al. (2012)
LTCC (Cacna1c) (exon 46–47) CTGTCCCCAAAGAGGGGCTT TCGCTTCAGACATTCAAGGT Jung et al. (2012)
NCX1 (Slc8a1) (exon 10–11) GAGACCTCGCTTCCCACTTC ATGCCCAGGAAGACGTTCACC Jung et al. (2012)
Jph2 (exon 4–5) AGGCTGGGGCCAAGAAAAAG CGATGTTCAGCAGGATCACCA Xu et al. (2012)
pan-Bin1 (exon 5–5/6) ACAATGACCTGCTCTGGATGG GCGACTTGATGTCAGGGAACT Gayi et al. (2018)
Dnm2 (exon 3–4) ACAGGAGATCGAAGTGGAGAC GGTCGATGAGGGTCAGGTTCAA Gayi et al. (2018)
Mtm1 (exon 4–5) GAGGCGCCACAAGTAGAGG GAAGGCGTATCTTGTGAGGA Gayi et al. (2018)

Gene-specific primers were designed based on previously described primer pairs, for measuring mRNA levels of the following transcripts: RyR2, LTCC, Jph2, pan-Bin1, Dnm2 and Mtm1. Primer pair specificity was controlled via dissociation curve analysis.