TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or phenotype | Reference |
|---|---|---|
| P. aeruginosa | ||
| PAO1 | Wild-type, elastolytic prototroph | 9 |
| PAO-R1 | lasR::Tcr; nonelastolytic derivative of strain PAO1 | 6 |
| PR1-E4 | lasR::Tcr; elastolytic mutant of strain PAO-R1 | This study |
| PG201 | Wild-type prototroph | 14 |
| PAO-JP2 | lasI rhlI double mutant derived from PAO1; Hgr Tcr | 21 |
| PAO-JP3 | lasR rhlR double mutant derived from PAO1; Hgr Tcr | 21 |
| Plasmids | ||
| pSW205 | Translational lacZ fusion vector that contains an origin of replication for both E. coli and P. aeruginosa; Apr | 7 |
| pTS400 | pSW205 containing a lasB′-lacZ translational fusion | 16 |
| pECP60 | pSW205 containing an rhlA′-lacZ translational fusion | 22 |
| pLP170 | Transcriptional lacZ fusion vector that contains an origin of replication for both E. coli and P. aeruginosa; Apr | 23 |
| pPCS1002 | pLP170 containing a rhlR′-lacZ transcriptional fusion | 22 |
| pLPRI | rhlI′-lacZ transcriptional fusion; a 904-bp NcoI fragment of strain PG201 chromosomal DNA containing 723 bp upstream of the rhlI translational start site was ligated into the SmaI site of pLP170 | This study |
| pPCS223 | lasI′-lacZ transcriptional fusion; a 407-bp fragment containing 288 bp upstream of the lasI translational start site, with PCR-engineered HindIII and BamHI restriction sites, was ligated into the HindIII-BamHI sites of pLP170 | This study |