Figure 5.

An overview of identified surface proteins on EVs from cell lines. (A) Comparison of all identified surface proteins from three cell lines and two EV fractions with EV databases. (B) Comparison of identified proteins from sEVs from each cell line from previous total proteomics analysis with surface proteins identified by surface labeling and MS analysis. (C) Biomarker selection based on common surface proteins from MDA-MB-231 and MCF7, not identified in MCF10A sEVs, was annotated with known disease associations from the DisGeNET database, and subsequently, proteins associated with cancer and BC were selected. The final list of potential BC EV surface markers was ranked based on LFQ intensity to prioritize more abundant proteins (Table 2).