Figure 5.

IFN-γ of hu-BLT NK and T cells when cultured with OCs and sAJ2. Hu-BLT mice NK and CD3 + T cells were isolated from splenocytes, and hu-BLT mice monocytes were isolated from bone marrow as described in the Material and Methods section. OCs were generated as described in the Materials and Methods section. NK cells (1 × 10⁶ cells/mL) were treated with a combination of IL-2 (1000 U/mL) and anti-CD16 mAbs (3 µg/mL) overnight. Cells were then cultured with OCs and sAJ2 (1:2:4; OCs:NK:sAJ2). CD3+ T cells were treated with rh-IL-2 (100 U/mL) and anti-CD3+ (1µg/mL)/anti-CD28 (3 µg/mL) for 18–20 h. Cells were then cultured with OCs and sAJ2 (1:2:4; OCs: CD3 + T:sAJ2). Cells were counted (A), and the supernatants were harvested on days 6, 9, and 12 from the co-cultures to determine the IFN-γ secretion level using single ELISA (B), which was determined per million of cells (C). The average of the two experiments is shown in the figure. **** (p value <0.0001), *** (p value 0.0001–0.001), ** (p value 0.001–0.01).