TABLE 1.
Cytokine production by splenocytes from mice immunized with rSAG1 alone or combined with IL-12
| Immunization regimen | Production of cytokine (pg/ml)a
|
||||
|---|---|---|---|---|---|
| IL-2 | IFN-γ | IL-4 | IL-6 | TNF-α | |
| Unstimulated control cells (RPMI) | |||||
| Vehicle | <10 | 400 ± 30 | 23.9 ± 9.0 | 211.4 ± 9.2 | 328.0 ± 7.5 |
| IL-12 | <10 | 500 ± 50 | <8 | 55.9 ± 9.0 | 68.3 ± 3.3 |
| rSAG1 | <10 | 200 ± 40 | 13.6 ± 1.6 | 81.5 ± 7.0** | 78.8 ± 9.9** |
| rSAG1–IL-12 | <10 | 1,500 ± 60*** | <8 | 302.5 ± 9.0** | 916.0 ± 7.8** |
| TLA stimulation | |||||
| Vehicle | 26.7 ± 2.0 | 1,600 ± 50 | 19.0 ± 4.0 | 146.9 ± 7.0 | 627.8 ± 7.7 |
| IL-12 | 30.1 ± 5.0 | 600 ± 70 | <8 | 92.2 ± 3.0 | 273.0 ± 13.0 |
| rSAG1 | 12.0 ± 2.3** | 200 ± 30** | 8.2 ± 0.0 | 111.3 ± 6.8 | 181.0 ± 0.0** |
| rSAG1–IL-12 | 33.1 ± 4.7* | 3,100 ± 20** | <8 | 158.2 ± 2.0 | 572.0 ± 9.3 |
a
Results are expressed as means ± standard deviations of triplicate wells. *, P < 0.05; **, P < 0.001 (Student’s t test for comparisons between vehicle control group and rSAG1 or rSAG1–IL-12 treatment group).