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. 2024 Jan 24;13(3):216. doi: 10.3390/cells13030216

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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shRNA−resistant SOX2 is unable to rescue suppression of sphere formation, invasion, and migration observed in shSOX2 LUAD cells. (A,B) The impaired sphere formation caused by shSOX2 is not restored by the reconstitution of SOX2 in LUAD cells. ‘+’ Dox indicates that 1 μg/mL Dox was treated. Representative images (top) and the quantification of TS number (bottom) are shown. TS formation assays were conducted using shMock, shSOX2, shSOX2/+Mock, and shSOX2/+SOX2 in A549 (A) and H358 (B) cells. Results from each independent experiment (Experiment 1,2 and 3) are presented as means ± SD (n = 3). (C,D) Reconstitution of SOX2 is unable to restore the reduced migration and invasion observed in shSOX2 LUAD cells. Migration (C) and invasion (D) assays using indicated A549 with or without Dox treatment. Means ± SD from three independent experiments are presented. ***, p < 0.001; **, p < 0.01 when compared with the shMock without Dox; ns, not significant; TS, tumor sphere. Scale bar: 100 μm.