Figure 7.

Proteomics analysis of the SDMA immunoprecipitated plasma sEV proteins from patients with early-stage PDAC and matched healthy subjects. Plasma sEV lysates (n = 18/group, pooled samples) from early-stage PDAC and healthy controls were immunoprecipitated with the SDMA antibody followed by proteomics analysis. (A) Western blot validation of immunoprecipitation by the SDMA antibody using PANC-1 cell lysates. (B) Venn-diagram showing numbers of arginine-methylated proteins that are shared or exclusively present in plasma sEV lysates from patients with early-stage PDAC (total 69 proteins) and healthy subjects (total 61 proteins). (C) Selected proteins that are exclusively arginine-methylated and closely associated with human cancer.