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. 2024 Jan 30;13(3):258. doi: 10.3390/cells13030258

Figure 8.

Figure 8

PGE2 contributed to GBM chemoresistance. OPN levels secreted in (A) T98G-, (B) U87MG-, and (C) GL44-sphere supernatants were assessed by ELISA upon stimulation with exogenous PGE2 and the drug combination CXB+TMZ for 72 h in the presence of macrophages. OPN immunoblotting assays of (D) T98G, (E) U87MG, and (F) GL44 treated as described above were normalized vs. β-Actin. CD44 immunoblotting assays of (G) T98G, (H) U87MG, and (I) GL44 treated as described above were normalized vs. β-Actin. Representative images are shown. Values are expressed as the fold increase versus CNTR (mean ± SEM) of two independent experiments. A one-way ANOVA with a post hoc Tukey’s test was used (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).