Figure 4.

Neighborhood analysis of the orthotopic PDAC model. (A) The heatmap shows the average proximity score for each pair of cell phenotypic clusters, averaged over all the acquired ROIs (8 ROIs). Positive (red) or negative (blue) values indicate that a specific pair of phenotypes is neighboring significantly more often or significantly less often, respectively, than expected from randomized placement, as described in Section 4. A 30 μm radius is considered for cell−to−cell proximity. (B) Tumor margin, as in panel (A), averaged over all the acquired ROIs (9 ROIs). A 30 μm radius is considered for cell−to−cell proximity. (C) Representative images showing the distribution of cells belonging to the monocyte/macrophage (cyan), CD8⁺ T cell (yellow), dendritic cell (green), neutrophil (cyan), CD4⁺ T cell (blue) and B cell (yellow) clusters with the tumor cell (magenta) cluster over the cell mask, in the tumor core and margin. (D) Representative images showing the arrangement of cells belonging to the endothelial (magenta) and lymphatic (cyan) clusters in relation to the stromal cluster (yellow) in the tumor core and tumor margin, white arrows: Endothelial, green arrowss: Lymphaic. Bar, 100 μm. n = 3 to 6 ROIs for each mouse (n = 4). Total acquired ROIs: core: n = 8; margin: n = 9.