Table 1.
Imaging mass cytometry antibody panel.
| Target | Clone | Metal | Source |
|---|---|---|---|
| Ly6G | 1A8 | 141Pr | Standard Biotools (San Francisco, CA, USA) |
| Vimentin | D21H3 | 143Nd | Cell Signaling (Danvers, MA, USA) |
| MHC–II | M5/114.15.2 | 145Nd | Invitrogen (Waltham, MA, USA) |
| CD45 | 30-F11 | 147Sm | Invitrogen |
| PanCK | AE1/AE3 | 148Nd | Standard Biotools |
| Fibrinogen | Polyclonal | 149Sm | Dako |
| αSMA | 1A4 | 151Eu | Sigma |
| CK19 | EPNCIR127B | 152Sm | Abcam (Cambridge, UK) |
| F4/80 | Cl:A3-1 | 153Eu | BioRad (Hercules, CA, USA) |
| PDGFRα | Polyclonal | 154Sm | R&D (Sunnyvale, CA, USA) |
| uPAR | Polyclonal | 155Gd | R&D |
| PDGFRβ | Polyclonal | 156Gd | R&D |
| E–cadherin | 24E10 | 158Gd | Standard Biotools |
| CD3 | 17A2 | 159Tb | Invitrogen |
| PV1 | MECA-32 | 160Gd | BD Pharmigen (San Diego, CA, USA) |
| CD4 | GK1.5 | 161Dy | Invitrogen |
| CD8 | 53-6.7 | 163Dy | Invitrogen |
| Lyve–1 | Polyclonal | 164Dy | Abcam |
| Desmin | Y66 | 165Ho | Abcam |
| CD31 | 2H8 | 166Er | Merck Millipore (Burlington, MA, USA) |
| Collagen IV | Polyclonal | 167Er | BioRad |
| CD206 | Polyclonal | 168Er | Abcam |
| Collagen I | Polyclonal | 169Tm | Standard Biotools |
| CD44 | IM7 | 171Yb | Standard Biotools |
| CD11b | M1/70 | 172Yb | Standard Biotools |
| ZO−1 | 1A12 | 173Yb | Invitrogen |
| CD103 | Polyclonal | 175Lu | R&D |
| B220 | RA3-6B2 | 176Yb | Invitrogen |