Figure 7.

Representative immunoblots show the effects of antiretrovirals on the expression of proteins regulating cell death. The antiretroviral cabotegravir induced apoptosis in SK-Mel-28 and FO-1 melanoma cells but not in the A375 melanoma cell line, as suggested by the high expression of the cleaved form of poly (ADP-ribose) polymerase (cPARP). Ferroptosis is induced in all cell lines, as suggested by a decrease in ferritin (FTH1) and/or glutathione peroxidase 4 (GPX4) expression and increased transferrin. Metalloproteinase 2 (MMP2) protein expression is sharply decreased in A375 FO-1, and SK-Mel-28 cell lines. Histograms represent the mean values ± S.D. of protein expression level measured by densitometry derived from three independent experiments and normalized with α-tubulin expression. All comparisons were performed vs. each control sample after data normalization; * p < 0.05; ** p < 0.01.