Table 1.

Examples of features of platelet-rich plasma (PRP) used for enrichment of biomaterials for tissue engineering applications.

Preparation Method	Platelet Activation	Platelet Count; Leukocyte Count	Application	Ref.
Centrifugation 1400 rpm, 15 min	3 freeze–thaw cycles (−80 °C and 37 °C)	1.9 × 106/μL; 1 NP	2 STE	[70]
Centrifugation 600× g, 5 min	CaCl2 solution	1.0 × 106/μL 1 NP	2 STE	[75]
Centrifugation 830× g, 8 min	CaCl2 solution	1.12 × 106/μL; 2.37 × 103/μL	2 STE	[100]
Centrifugation 200× g, 10 min 1500× g, 20 min	collagen	4–4.5 × 105/μL; 1 NP	3 NTE	[101]
Centrifugation 650× g, 30 min 1500× g, 15 min	CaCl2 solution	1.5 × 106/μL; 1 NP	3 NTE	[102]
Centrifugation 300× g, 10 min 1200× g, 15 min	1 NP	1.0 × 106/μL; 1 NP	4 CTE	[103]
Centrifugation 160× g, 10 min 400× g, 10 min	1 NP	3 × higher than in whole blood; 1 × higher than in whole blood	4 CTE	[104]
Centrifugation 200× g, 30 min 2000× g, 10 min	thrombin	3.23 × 106/μL; 1 NP	4 CTE	[71]
Centrifugation 200× g, 20 min 400× g, 8 min	1 NP	8.96 × 105/μL; 1 NP	5 BTE	[105]
Centrifugation 1000× g, 10 min	1 NP	~4.02 × 105/μL; ~3.31 × 103/μL	5 BTE	[106]
Centrifugation 2400 rpm, 10 min 3600 rpm, 15 min	1 NP	Approx. 7 × higher than in whole blood; 1 NP	5 BTE	[74]

¹ NP—not provided; ² STE—skin tissue engineering; ³ NTE—nerve tissue engineering; ⁴ CTE—cartilage tissue engineering; ⁵ BTE—bone tissue engineering.