Figure 2.

Workflow for antibody microarray analysis of bdEP isolated from Control and AD cases. Plasma samples of Control and AD cases were pooled, bdEP isolated, and further subjected to sample preparation prior to antibody microarray analysis. Globally normalized signal intensity obtained for each target was compared between the batches of Control and AD cases by paired t-test. Proteins that presented significantly different signal intensities were further characterized by Gene Ontology and Reactome pathway analysis. Interaction networks based on the %CFC between Controls and ADs were constructed for altered proteomes and/or phosphoproteomes. In addition, a volcano plot was constructed for the identification of most relevant proteins, and a heatmap was constructed to assess bdEP phosphoproteins discrimination of Controls and AD cases. Abbreviations: AD, Alzheimer’s disease; C, Controls; bdEP, blood-derived extracellular particles.