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. 2024 Feb 3;25(3):1873. doi: 10.3390/ijms25031873

Figure 8.

Figure 8

Identification of regulatory relationship between SlNAC1 and SlERF109-like. (A) Interaction between SlNAC1 and SlERF109-like. (a) Model of nLuc/cLuc, SlNAC1-nLuc/cLuc and SlERF109-like-nLuc/cLuc constructs. (b) Firefly luciferase complementation assays of nLuc/cLuc, SlNAC1-nLuc/cLuc, and SlERF109-like-nLuc/cLuc in six-week-old tobacco leaves. (B) Y2H assay of interactions between SlNAC1 and SlERF109-like. Positive colonies signify strong interactions between SlNAC1 and SlERF109-like. (C) SlNAC1 can bind directly to the SlACO1 promoter while SlERF109-like cannot. (a) Scheme of the promoter of SlACO1. Bar segments were analyzed using the PlantCARE database and are separated by solid lines (F1, F2). (b) Identification of SlNAC1 bound to the SlACO1 promoter with the yeast one-hybrid assay.