Figure 2.

Representative images from the molecular layer of the dentate gyrus in the hippocampus showing pre-processing and thresholding strategy. Representative image of IBA1 immunoreactivity prior to thresholding; scale bar 20 µm (A). The two threshold levels set for images display total cells (B) and somas only (C). The analyze particles function was used to quantify the area for each image, as represented in (B) and (C) by a blue overlay.