TABLE 1.
Determination of MoPn levels in lung by ELISA or culture at days 5 and 15 after infection
| Mouse group | MoPn level detected bya:
|
|
|---|---|---|
| ELISA (ng/lung) | Culture (IFU/lung) | |
| Day 5 | ||
| Expt 1 | ||
| B6129F2/J | ||
| Treated with rat IgG | 2,409 ± 214 | NDb |
| Treated with anti-IL-10 antibody | 1,682 ± 219† | ND |
| IL-6−/− | ||
| Treated with rat IgG | 5,782 ± 717* | ND |
| Treated with anti-IL-10 antibody | 1,778 ± 215** | ND |
| C57BL/6J | 1,642 ± 172†† | ND |
| Expt 2 | ||
| B6129F2/J | ND | (6.0 ± 1.4) × 103 |
| IL-6−/− | ND | (27.5 ± 8.3) × 103*** |
| Day 15 | ||
| Expt 3 | ||
| B6129F2/J | 32,230 ± 6,040 | (12.6 ± 4.6) × 104 |
| IL-6−/− | ||
| Treated with rat IgG | 62,520 ± 6,320§ | (39.4 ± 18) × 104# |
| Treated with anti-IL-10 antibody | 35,689 ± 7,467∥ | (17.8 ± 6.4) × 104‡ |
| Expt 4 | ||
| B6129F2/J | 10,300 ± 4,700 | ND |
| IL-6−/− | 89,400 ± 24,700‡‡ | ND |
a
Data are expressed as means ± standard errors. All statistics were calculated according to the Mann-Whitney test. *, P < 0.008 versus B6129F2/J treated with rat IgG (two-tailed test); †, P < 0.08 versus B6129F2/J treated with rat IgG (two-tailed test); **, P < 0.008 versus IL-6−/− treated with rat IgG (two-tailed test); ††, P > 0.2 versus B6129F2/J treated with rat IgG; ***, P = 0.56 versus B6129F2/J; §, P < 0.004 versus B6129F2/J (two-tailed test); ∥, P > 0.99 versus B6129F2/J (two-tailed test); #, P < 0.06 versus B6129F2/J (two-tailed test); ‡, P > 0.4 versus B6129F2/J (two-tailed test); ‡‡, P < 0.008 versus B6129F2/J.
b
ND, not done.