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. 2024 Jan 25;29(3):597. doi: 10.3390/molecules29030597

Figure 1.

Figure 1

Effects of AT root fractions on migration and tube formation of HUVECs. (A) The migratory ability of HUVECs was assessed via a transwell assay after treatment with HAT, EAT, and BAT (50 μg/mL). The migrated cells were photographed (×100 magnification; scale bar = 200 μm). (B) Relative migration compared to control cells was determined by counting the stained cells. (C) Tube formation of HUVECs after treatment with HAT, EAT, and BAT (50 μg/mL) was photographed (×50 magnification; scale bar = 0.4 μm). (D) Quantification of tube formation was conducted by counting the number of loops. Statistical analyses were performed using one-way ANOVA followed by Tukey’s post hoc test. * p < 0.05, *** p < 0.001 vs. control cells. BAT—butanol fraction of Adenophora triphylla var. japonica root extract; EAT—ethyl acetate fraction of Adenophora triphylla var. japonica root extract; HAT—hexane fraction of Adenophora triphylla var. japonica root extract; HUVEC—human umbilical vein endothelial cell.